1. Prepare materials for mass production of Metarhizium anisopliae
Equipment:
1. Pressure cooker/autoclave
2. Inoculating chamber/Laminar Flow Hood
Supplies:
- Artificial culture media
- Polypropylene plastic bag (9 x 16) or any heat-resistant plastic bag
- Inoculating loop/inoculating needle
- Glass syringe, 10 ml (dispenser)
- Detergent (all-purpose)
- Ethyl alcohol
- Denatured alcohol
- Glass stirring rod
- Chlorox
- Cotton
- Rubber bands
- Alcohol lamp
- Spatula
- Hand sprayer
- Lighter/match
- PVC Ring
- Palay/corn
Glassware:
- 500 ml and 1000 ml beakers
- 250 ml and 500 ml flasks
- Test tubes
- Petri dishes
2. Prepare and inoculate artificial culture media (See techno tip on How to Prepare and Inoculate Artificial Culture Media)
3. Prepare palay substrates (See techno tip on How to Prepare Palay Substrates)
4. Inoculate palay substrates (See techno tip on How to Inoculate Palay Substrates)
